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. 2020 Jan 13;43(3):886–896. doi: 10.3892/or.2020.7460

Figure 4.

Figure 4.

Apoptotic changes of cell nuclei induced by CK in MG63 and U2-OS cells detected by TUNEL assays. (A and B) Cells were treated with or without CK (20 µM) and cell nuclei were stained with DAPI and TUNEL assay solution and observed under a fluorescence microscope. Normal nuclei exhibited regular elliptical shapes and were stained blue by DAPI; apoptotic nuclei presented as condensed or fragmented and were stained green by TUNEL assay solution. Compared with the control group, the CK group exhibited significant apoptosis. Data are expressed as the means ± SEM. All experiments were repeated three times. *P<0.05 vs. the NC group. CK, ginsenoside compound K; NC, normal control.