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. 2020 Jan 13;11(2):775–790. doi: 10.1364/BOE.375678

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Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.

Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.

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Fig. 3. — Dependency of localization precisions on z-position and defocus setting $Γ$ , for NAs of 1.7 (a) and 1.49 (b), respectively. The precisions are stated in decadic log-scale. PSF images for 15 different $Γ$ /z combinations are shown on the right.