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. 2020 Jan 13;11(2):775–790. doi: 10.1364/BOE.375678

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Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.

Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.

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Fig. 4. — Localization precisions for x- and z-dipole emitters for a defocus setting of $Γ$ =500 nm. The inset images in (b) show the respective dipole images for different distances to the coverslip. (signal=2000 photons, background level=100 photons per pixel, wavelength=670 nm, pixel size=115 nm, image size=15×15 pixel, NA=1.7)