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. 2020 Feb 24;58(3):e01555-19. doi: 10.1128/JCM.01555-19

FIG 1.

FIG 1

Differential growth in the combined CAST/eCAST test. Colistin-resistant isolates showed growth in the colistin agar-spot screening test (CAST) (plate A: Mueller-Hinton agar with 3 μg/ml COL). Of these isolates, only MCR producers did not grow in 1 mM EDTA colistin agar-spot screening test (eCAST) (plate B: Mueller-Hinton agar with 3 μg/ml COL + 1 mM EDTA). In contrast, mcr-negative strains harboring other resistance mechanisms also grew in these plates. A control plate (plate C: Mueller-Hinton agar with 1 mM EDTA) was used as a growth control for each isolate. Sections 1 to 10, mcr-positive COL-resistant isolates; 11 to 16, mcr-negative COL-resistant isolates; 17 to 21, mcr-negative COL-susceptible isolates.