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N. gonorrhoeae-simulated infections; limit of detection using Nanopore sequencing. (A) The proportion of sequenced reads classified as human, bacterial, or viral. (B) The proportion of bacterial bases classified as N. gonorrhoeae, and (C) the proportion of the NCCP11945 N. gonorrhoeae reference genome covered at ≥10-fold depth. For WHO V (orange markers), the actual spike concentration achieved was lower than the targeted concentration (see Fig. S3).
