TABLE 2.
Comparison of rates of enteropathogen detection by LDTs and the GIP assay
| QIAstat-Dx GIP target | No. of results |
|||
|---|---|---|---|---|
| LDT+/GIP+ | LDT+/GIP− | LDT−/GIP+ | LDTNPc /GIP+ | |
| Bacterial | ||||
| Clostridium difficile toxin A/B | 13 | 1 | ||
| EAECa | 13 | |||
| EPECa | 17 | |||
| ETEC LT/STa | 4 | |||
| STEC stx1/stx2 | 5 | |||
| STEC O157:H7a | 1 | |||
| EIECd /Shigella | 6 | |||
| Pathogenic Campylobacter spp. | 9 | 3 | ||
| Plesiomonas shigelloides | 4 | 1 | 1a | |
| Salmonella spp. | 7 | |||
| Vibrio choleraea | ||||
| Vibrio parahaemolyticusa | ||||
| Vibrio vulnificusa | ||||
| Yersinia enterocolitica | 2 | 3 | ||
| Parasites | ||||
| Cyclospora cayetanensis | 2 | 1 | ||
| Cryptosporidium spp. | 6 | 1 | 1 | |
| Entamoeba histolytica | 2 | 2 | ||
| Giardia lamblia | 7 | 1 | ||
| Viruses | ||||
| Adenovirus F40/41 | 3 | |||
| Astrovirus | 5 | 1 | ||
| Norovirus GI + norovirus GIIb | 16 | 1 | ||
| Rotavirus | 6 | |||
| Sapovirus (I, II, IV, V) | 4 | |||
| Total | 97 | 10 | 6 | 36 |
a
Detection of enteroaggregative E. coli (EAEC), enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), Shiga-like-toxin-producing E. coli (STEC) O157:H7, and Vibrio spp. are not part of the routine diagnostics at both institutes and is therefore not tested using LDTs. In addition, RUMC-Nijmegen does not use a LDT for the detection of P. shigelloides.
b
The LDT performed at RUMC-Nijmegen does not differentiate between norovirus GI and norovirus GII. In total, six norovirus GI and 11 norovirus GII were detected using GIP testing.
c
NP, not performed.
d
EIEC, enteroinvasive E. coli.