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. 2020 Feb 24;58(3):e01591-19. doi: 10.1128/JCM.01591-19

TABLE 2.

H. pylori isolate Etest MIC values and resistance mutations/genes for amoxicillin, clarithromycin, levofloxacin and tetracycline, metronidazole and rifampina

SId no. Isolate identifier MIC (μg/ml)
 Mutation for resistance to:
 Infection typec
AMX CLR LVX TET MET RIF AMX (pbp1A, pbp2, pbp3) CLR (23S rRNA) LVX (gyrA) TET (16S rRNA) MET (rdxA) RIF (rpoB)
1 MHP1 0.016 0.032 0.064 0.032 0.38 1 P
2 MHP2 0.023 0.094 0.047 0.023 0.047 0.125 R
3 MHP3 0.016 0.023 0.023 0.032 0.5 0.5 P
4 MHP4 0.032 12 <32 0.064 0.19 1 A2143G N87K R
5 MHP5 0.016 0.016 0.006 0.023 0.032 0.19 P
6 MHP6 0.016 0.023 0.016 0.032 0.25 0.50 P
7 MHP8 0.016 0.016 >32 0.125 >256 0.5 N87K R16H P
8 MHP9b 0.032 >256 >32 0.064 >256 1 A2143G N87K 75 stop R
9 MHP10 0.064 3 >32 0.032 >256 A2143G N87K 50 stop R
10 MHP11 256 0.016 >32 0.25 0.75 0.5 pbp1A, I259T; pbp3, D2N, A50S, F490Y, A541T D99N P
11 MHP12 0.19 >256 >32 0.032 0.75 0.5 pbp2, T498S A2143G N87I 112 stop R
12 MHP13 0.064 0.016 0.125 0.5 >256 0.19 112 stop R
13 MHP15 0.032 0.023 0.125 0.5 0.19 2 112 stop R
14 MHP16 0.064 24 >32 0.032 >256 0.38 A2143G D91Y 197 stop P
15 MHP17 0.047 0.023 >32 0.064 >256 >32 N87I 119 stop K2691R R
16 MHP18 0.023 0.032 0.125 0.023 >256 3 76 stop R
17 MHP19 0.032 >256 0.19 0.047 1.0 0.5 A2143G P
18 MHP20 0.016 0.016 >32 0.125 >256 0.5 N87K P
19 MHP22 0.25 0.125 0.25 0.125 0.125 0.38 pbp1A, N107R, A201V, V250I, S543T; pbp3, V374I P
20 MHP23 0.016 >256 >32 0.125 0.016 4 A2143G N87I D221N, F971Y, K1229N, V2664M P
21 MHP28 0.023 0.016 0.047 0.094 >256 0.38 A2143G A926G R16H, H99R R
22 MHP29 0.016 0.047 >32 0.19 0.064 0.5 N87I P
23 MHP30 0.023 0.032 0.094 0.125 0.125 0.125 P
24 MHP31 0.016 >256 8 0.023 0.38 2 A2143G D91N R
25 MHP32 0.094 0.125 >32 0.75 >256 3 N87I A926G R
26 MHP33 0.016 0.064 >32 0.125 0.032 1 N87I R
27 MHP34 0.016 0.047 >32 0.19 >256 1.5 A2143G D91N R16H R
28 MHP35 0.016 0.016 >32 0.094 0.125 1 P
29 MHP36 0.023 >256 0.047 0.032 >256 1 A2142G R16H, V57A R
30 MHP37 0.032 3 0.094 0.19 >256 1.5 A2143G N14T, R16H, W209Q, L210N P
31 MHP38 0.047 <0.016 4 0.094 0.094 0.125 D91G A926G 53 stop P
32 MHP39 0.016 0.047 >32 0.032 0.38 1.5 N87I P
33 MHP40 0.016 >256 >32 0.023 >256 3 A2142G D91Y 143 stop R984C, T1042A, T1965M, N2219S R
34 MHP41 0.016 0.064 0.125 0.023 0.016 0.5 P
35 MHP42 0.064 0.023 >32 0.064 0.032 12 N87I M415V, I2021V P
36 MHP43 0.023 0.016 >32 0.064 0.016 >32 N87I I125T, T897A, Q1010R P
37 MHP44 0.016 0.047 >32 0.064 >256 0.38 N87I A926G R16H R
38 MHP47 0.016 0.50 0.064 0.023 >256 1 75 stop R
39 MHP50 0.047 0.016 0.094 0.125 >256 1 A2142G N87K 72 stop P
40 MHP51 0.023 0.016 0.125 0.047 0.19 0.5 P
41 MHP52 0.047 >256 >32 0.047 >256 2 A2142G N87K 72 stop R
42 MHP54 0.023 >256 >32 0.094 >256 0.38 A2143G N87I 90 stop R
a

Isolates with phenotypically resistant MIC values are highlighted in light gray shading. Discrepancies among WGS results compared to phenotypic resistance are highlighted in dark gray shading. Blank cells indicate that no mutations associated with resistance were found in the 23S rRNA gene for clarithromycin, gyrA and gyrB for levofloxacin, pbp1, pbp2, and pbp3 for amoxicillin, 16S rRNA for tetracycline, or rdxA for metronidazole. AMX, amoxicillin; CLR, clarithromycin; LVX, levofloxacin; TET, tetracycline; MET, metronidazole; RIF, rifampin.

b

MHP9 is a heteroresistant isolate comprised of strain MHP9C (resistant to clarithromycin) and MHP9L (resistant to levofloxacin).

c

P, primary; R, recurrent.

d

SI, specimen isolate.