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. 2020 Feb 24;58(3):e01566-19. doi: 10.1128/JCM.01566-19

TABLE 2.

Stool samples obtained from patients with diarrhea, bloody diarrhea, or HUS

Sample ID Clinical diagnosisa Age Serotypeb Genotypic characterizationc
FFStxd
eae/ehx stx Vero cell cytotoxicity assay Stx Quik Chek assay VHH-based ELISA
1 HUS 3 mo O145:NM +/+ stx2a ND Pos (Stx2) Pos
2 BD 6 yr O145:NM +/+ stx2a ND Pos (Stx2) Pos
3 BD 13 mo O157:H7 +/+ stx2a/2c ND Pos (Stx2) Pos
4 BD 1 yr O145:NM +/+ stx2a ND Pos (Stx2) Pos
5 HUS 41 mo O59:H19 −/+ stx2a Pos Pos (Stx2) Pos
6 UFS 10 mo O26:H11 +/+ stx1a ND Pos (Stx1) Neg
7 UFS 1 mo ONT:HNT +/+ stx1a ND Neg Neg
8 HUS 20 mo O157:H7 +/+ stx2a/2c ND Pos (Stx2) Pos
9 D 7 yr O121:HNT +/+ stx2a ND Pos (Stx2) Pos
10 D 11 yr O145:NM +/+ stx2a ND Pos (Stx2) Pos
11 D 10 mo O145:NM +/+ stx2a ND Pos (Stx2) Pos
12 HUS 18 mo O145:NM +/+ stx2a Neg Neg Neg
13 HUS 2 yr O157:H7 +/+ stx2a/2c Neg Neg Neg
14 HUS 11 mo O157:H7 +/+ Neg Neg Neg Neg
15 HUS 21 mo ONT:HNT ND Neg Neg Neg Neg
16 HUS 60 mo ONT:HNT ND Neg Neg Neg Neg
17 D 3 yr ONT:HNT ND Neg ND Neg Neg
18 BD 2 yr ONT:HNT ND Neg ND Neg Neg
19 D 2 yr ONT:HNT ND Neg ND Neg Neg
20 BD 11 mo ONT:HNT ND Neg ND Neg Neg
21e HUS 2 yr ONT:HNT ND Neg Pos Neg Pos
22 BD 3 mo ONT:HNT ND Neg ND Neg Neg
a

HUS, hemolytic-uremic syndrome; BD, bloody diarrhea; D, diarrhea; UFS, unspecific febrile syndrome.

b

Serotype was determined by seroagglutination using specific antisera. ONT, O polysaccharide not typified; HNT, flagellar H antigen not typified; NM, not motile.

c

eae, ehxA, and stx gene(s) detection by PCR. ND, not determined.

d

Free fecal Shiga toxin (FFStx) detection in stool samples. Pos, positive; Neg, negative; ND, not determined; VHH-based ELISA, biotin-streptavidin VHH-based capture ELISA with spectrophotometric detection.

e

Bacterial isolation, negative; immunomagnetic separation for O157, negative; multiple PCR for rfbO157/stx1/stx2, negative; PCR for diarrheagenic Escherichia coli including enteropathogenic E. coli (eae), enteroaggregative E. coli (aggR), enteroinvasive E. coli (ipaH), and enterotoxigenic E. coli (heat-labile enterotoxin/heat-stable enterotoxin), negative; Chemlis E. coli O157, O145, and O121 (Chemtest Argentina S.A., detection of specific IgM and IgG antibodies in serum sample), negative.