TABLE 3.

Substances tested to assess interference in the performance of the ResistancePlus MG FleXible assay

Sample matrix	Class/substance	Product name	Test concn	Assay result for:a
				M. genitalium-negative samples	M. genitalium-positive samples (M6604 strain)
Urine	Whole blood	NAe	0.4% (vol/vol)b	ND	D
Urine	Semen	NA	5.0% (vol/vol)	ND	D
Urine	Mucus	Mucin	0.8% (wt/vol)	ND	D
Urine	Antibiotic	Azithromycin	1.8 mg/ml	ND	D
Urine	Analgesic	Paracetamol	3.2 mg/ml	ND	D
Urine	Intravaginal hormones	Progesterone; estradiol	7 mg/ml Progesterone + 0.07 mg/ml beta estradiol	ND	D
Urine	Leukocytes	NA	105 cells/ml	ND	D
Urine	Albumin	Bovine serum albumin	10 mg/ml	ND	D
Urine	Glucose	NA	10 mg/ml	ND	D
Urine	Bilirubin	NA	0.18 mg/mlc	ND	D
Urine	Acidic urine (pH 4.0)	Urine + N-acetyl-l-cysteine	pH 4.0	ND	D
Urine	Alkaline urine (pH 9.0)	Urine + ammonium citrate	pH 9.0	ND	D
Swab	Over-the-counter vaginal products and contraceptives	Vagisil anti-itch crème (1.0 oz)	0.25% (wt/vol)	ND	D
Swab	Over-the-counter vaginal products and contraceptives	K-Y Jelly (4.0 oz)	0.25% (wt/vol)	ND	D
Swab	Over-the-counter vaginal products and contraceptives	Options Gynol II vaginal contraceptive gel	0.25% (wt/vol)	ND	D
Swab	Over-the-counter vaginal products and contraceptives	Walgreens clotrimazole vaginal cream (1.5 oz)	0.25% (wt/vol)	ND	D
Swab	Over-the-counter vaginal products and contraceptives	Vagi-gard douche	0.25% (wt/vol)	ND	D
Swab	Over-the-counter vaginal products and contraceptives	Vagisil ProHydrate natural feel internal moisturizing gel (0.2-oz 8 pack)	0.25% (wt/vol)	ND	D
Swab	Over-the-counter vaginal products and contraceptives	Vagisil daily intimate deodorant powder (8.0 oz)	0.10% (wt/vol)d	ND	D
Swab	Deodorant and powders	Summer's Eve deodorant spray (2.0 oz)	0.25% (vol/vol)	ND	D
Swab	Hemorrhoidal cream	Preparation H hemorrhoidal cream (0.9 oz)	0.25% (wt/vol)	ND	D
Swab	Prescription-only medicines	Estrace (Estradiol vaginal cream, USP 0.01%)	0.25% (wt/vol)	ND	D

^aD, output result “M. genitalium detected; 23S rRNA mutation detected”; ND, output result “M. genitalium not detected; 23S rRNA mutation not detected.”

^bInterference may be observed in samples containing >0.4% whole blood.

^cInterference may be observed in samples containing >0.18 mg/ml bilirubin.

^dInterference may be observed in samples containing >0.1% wt/vol Vagisil intimate powder.

^eNA, not applicable.