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. 2020 Feb 21;13:1613–1624. doi: 10.2147/OTT.S226300

Figure 4.

Figure 4

circHIPK3 regulated the proliferation and migration of GC cells. (A) Knockdown efficacy of circHIPK3 in BGC-823 and MKN45 cells was determined by qRT-PCR. (B) circHIPK3 promotes the proliferation of BGC-823 and MKN45 cells shown by the CCK8 assays. (C) Clone formation shows that circHIPK3 promotes the proliferation of BGC-823 and MKN45 cells. Scale bar,15mm. (D) Observation of DNA synthesis of BGC-823 and MKN45 cells transfected with si-circHIPK3 vectors by EdU assay. Scale bar,100μm. (E) Determination of cell invasive potential of BGC-823 and MKN45 cells transfected with si-circHIPK3 vectors by Transwell assay. Data are the means±SD of three experiments. *P<0.05; P**<0.01; ****P<0.0001. Scale bar, 100μm.