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Western blotting for PTEN, PI3K (P-PI3K 110α), AKT (P-AKT, Ser 473), NF-κB (P-NF-κB, Ser 536), XIAP, and P53. Cells were treated with Δ-Ru1 (20 μM) alone, Dox (2 μM) alone or in combination for 24 h, and immunoblotted with the indicated antibodies. P- represent phosphorylation. (A) The expression of PTEN. (B) The expression of PI3K/P-PI3K and AKT/P-AKT. (C) The expression of NF-κB and P-NF-κB. (D) The expression of XIAP. (E) The expression of P53.
