Figure 2.

Soybean oil affects the hypothalamic transcriptome. A, Heat map of differential hypothalamic gene expression (RNA sequencing [RNA-seq]) between mice fed vivarium (Viv) chow, CO, SO + CO, and PL + CO diets for 24 weeks. Color bar indicates level of gene expression relative to the whole data set. Top, genes upregulated in Viv chow vs the 3 high-fat diets. Bottom, genes downregulated in Viv chow. Arrows, genes mentioned in text. Asterisk, Kcng1, the only gene significantly different between SO + CO and PL + CO. B, Gene ontology showing categories of molecular function affected by diet. C, Absolute expression levels from RNA-seq of indicated diets. DEG, differentially expressed genes; RPKM, reads per kilobase per million. Statistically different from ^#CO, *Viv chow, ^@PL + CO. CO diets enriched in conventional soybean oil (SO + CO), genetically modified Plenish (PL + CO), and stigmasterol (ST + CO).