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. 2020 Jan 8;161(2):bqz044. doi: 10.1210/endocr/bqz044

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© Endocrine Society 2020.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Dietary soybean oil (SO) dysregulates hypothalamic gene expression and plasma levels of oxytocin. C57BL/6N male mice fed Viv chow, CO, SO + CO, PL + CO, or ST + CO diets for 17 to 28 weeks. A, Transcript levels of Oxt, expressed relative to β-actin (Actb) measured in hypothalamic homogenates using reverse transcriptase-quantitative polymerase chain reaction and expressed relative to levels in CO using the Pfaffl method (N = 3-6/diet). B, Peripheral oxytocin peptide levels measured via EIA, (N = 3-10/diet). Statistically different from: ^#CO; ^@@@PL + CO; ^{^}SO + CO and PL + CO (single symbols, P < .05; triple symbols P < .001) using 1-way analysis of variance (ANOVA) followed by Tukey post hoc analysis for A and one-way ANOVA followed by Bonferroni post hoc analysis for B.