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. 2019 Dec 23;219(2):e201903062. doi: 10.1083/jcb.201903062

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© 2019 Ci et al.

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Figure S3. — NS1 mutations in its conserved hydrophobic regions. (A) Alignment of hydrophobic regions among flavivirus NS1 sequences. Multiple hydrophobic residues in these regions are highly conserved. (B and C) Purification of the Myc-tagged WT and mutated ZIKV NS1 proteins. Purified NS1 proteins were analyzed by SDS-PAGE and Coomassie Blue staining (B) or Western blotting using an anti-Myc antibody (C).