Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 27;219(2):e201904048. doi: 10.1083/jcb.201904048

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Grey et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 5. — IRE1β interacts with IRE1α. (A) IRE1α and IRE1β proteins were assayed by immunoblot with anti-IRE1α or anti-FLAG antibodies, respectively, for lysates or samples immunoprecipitated with anti-FLAG from HEK293^doxIRE1β cells treated with Dox and Tg as indicated. Blots are representative of three independent experiments. (B) Oligomerization of endogenous IRE1α was assayed by gel filtration fractionation of lysates from HEK293^doxIRE1β cells and immunoblot with anti-IRE1α antibody. Fraction band intensities are plotted relative to the band intensity for the input sample, which was included on each gel, and normalized to fractions with the highest relative intensity (assigned a value of 1) and lowest relative intensity (assigned a value of 0). Symbols represent mean ± range for two independent experiments.