Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 27;219(2):e201904048. doi: 10.1083/jcb.201904048

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Grey et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure S2. — IRE1β suppresses XBP1 transcriptional activity. Supports Fig. 2. (A) HEK293T cells were cotransfected with UPRE-luciferase reporter and indicated plasmids, treated with Tg for 8 h, and assayed for luciferase activity. Activity is plotted relative to mock-transfected cells without Tg (n = 3). (B and C) Spliced XBP1 mRNA (B; n = 3) and XBP1 protein levels (C; n = 5) in HEK293T cells with control plasmid or IRE1β expression plasmid and treated with Tg for 4 h. Bars and error bars represent mean values ± SEM; significance is indicated by asterisks (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001).