Figure S2.

Silencing expression of NuMA1 or 4.1B does not affect AIS maintenance in hippocampal neurons. (a) Hippocampal neurons were transfected with Luc-shRNA, NuMA1-shRNA #1, NuMA1-shRNA #2, 4.1B-shRNA #1, or 4.1B-shRNA #2 at DIV9 and immunostained at DIV16 for GFP, AnkG, and NF186. Arrowheads in the AnkG-labeled panels indicate AIS. Scale bars = 5 µm. (b) Quantification of the staining pattern for AnkG in shRNA-transfected neurons. Mean ± SEM is shown (the number of neurons analyzed is indicated). No statistical comparison was performed.