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. 2019 Nov 21;217(2):e20190668. doi: 10.1084/jem.20190668

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© 2019 Majumder et al.

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Figure 4. — MHC mutant cells incompletely stimulate allogenic CD4 T cells to divide. Freshly isolated human CD4⁺ T cells were labeled with CTV and cocultured with Raji cells, RJ2.2.5 cells, or Δ21 cells for 6 d. (A) Histograms of CTV-labeled cells, set to observe T cells that divided at least once and lost CTV label. Negative (Neg) control in which CD4 T cells were incubated without stimulation. Positive (Pos) control in which CD4⁺ T cells were incubated with anti-CD3/CD28 beads. (B) Summation of data in A. Peripheral blood samples were isolated from five healthy subjects, and the experiment was performed in groups of two and three. Data represent mean ± SD. Subject samples were compared across all stimulations using a repeated measures one-way ANOVA, with the Greenhouse–Geisser correction followed by a Tukey’s post hoc test. *, P ≤ 0.05; **, P ≤ 0.01.