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. 2019 Nov 14;217(2):e20190860. doi: 10.1084/jem.20190860

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© 2019 du Pré et al.

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Figure 2. — Analysis of B cell development in 14E06 KI mice. (A) Percentage of TG2-specific B cells as detected by anti-14E06 antibody or mTG2 tetramers in Tgm2^+/+ and Tgm2^−/− 14E06 KI mice. Data are pooled from six independent experiments and show mean ± SD. (B and C) Analysis of B cell subsets in spleen. (B) Representative plots of spleen B220⁺ B cells stained for CD23 and CD21/35 expression to identify immature/T1 (CD23⁻CD21⁻), follicular/T2 (CD23⁺CD21⁺), and marginal zone (CD23⁻CD21^hi) subsets. (C) Pooled data from at least four independent experiments. Data are mean ± SD. (D and E) Analysis of developing B cells in bone marrow. (D) Representative plots of bone marrow B220⁺ B cells stained for anti-IgD and anti-IgM to identify pre-B cells (IgM⁻IgD⁻), immature and transitional B cells (IgM⁺IgD^−/lo), and mature recirculating (IgM⁺IgD⁺) B cells in WT C57Bl/6, Tgm2^+/+, and Tgm2^−/− 14E06 KI mice. (E) Data are pooled from at least four independent experiments and show mean ± SD. (F) Representative plots of spleen B220⁺ B cells stained with mTG2 tetramers and an anti-λ L chain antibody. *, P < 0.05, **, P < 0.0005, as determined by Student’s t test (C and E). Flow plots are representative of at least four independent experiments (B, D, and F). For C57Bl/6 control mice, we used either commercially obtained C57Bl/6 from Janvier Laboratoires or nontransgenic littermates from in house breedings. ns, not significant.