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. 2019 Nov 14;217(2):e20190860. doi: 10.1084/jem.20190860

Figure 7.

Figure 7.

Self-reactive TG2-specific B cells respond to T cell help. (A) Schematic representation of the 2W1S experiment. WT C57Bl/6 mice were immunized i.p. with 50 µg 2W1S peptide (pept.) in CFA 7 d before adoptive transfer of TG2-specific B cells from Tgm2+/+ 14E06 KI mice. The next day, mice were immunized i.p. with 100 µg TG2-2W1S fusion protein or TG2 alone in IFA. (B) Serum IgG anti-TG2 antibodies were measured at day 12 after TG2/TG2-2W1S administration. Data are mean ± SEM (n = 3/group) and representative of two independent experiments. Imm., immunized. (C) Schematic representation of the TG2-gluten experiment. TG2-specific B cells obtained from Tgm2+/+ 14E06 KI HLA-DQ2.5 mice and gluten-specific T cells obtained from TCR-glia-α2 HLA-DQ2.5 mice were adoptively transferred to HLA-DQ2.5 recipient mice. The next day, mice were immunized with 100 µg TG2 fused to a deamidated gluten T cell epitope (TG2-33merEEE) or with TG2 alone in PBS. (D) Serum IgG anti-TG2 antibodies were measured at day 6 after TG2/TG2-33merEEE administration. Data are mean ± SEM (n = 3/group) and representative of at least four independent experiments.