Table 2. Characteristics of Dnah17+/+, Dnah17+/M, and Dnah17M/M male mice.
| Parameters | Dnah17+/+ | Dnah17+/M | Dnah17M/M |
|---|---|---|---|
| Body weight (g) | 27.0 ± 0.5 | 26.1 ± 1.0a | 26.3 ± 0.8a |
| Testis weight (mg) | 164.5 ± 3.3 | 167.4 ± 0.4a | 176.2 ± 8.9a |
| Testis/body weight ratio (10−3) | 6.1 ± 0.1 | 6.4 ± 0.2a | 6.7 ± 0.2a |
| Fertility | |||
| No. of fertile/total mice | 3/3 | 3/3 | 5/5 |
| Pups per fertile pair | 24.7 ± 0.3 | 24.2 ± 0.2a | 8.7 ± 0.5*** |
| Semen parameters | |||
| Sperm count (107) | 1.4 ± 0.1 | 1.4 ± 0.1a | 1.3 ± 0.1a |
| Motile sperm (%) | 76.7 ± 1.2 | 72.7 ± 0.9a | 19.8 ± 3.4*** |
| Progressively motile sperm (%) | 32.7 ± 0.3 | 35.0 ± 1.2a | 9.2 ± 2.0*** |
| Sperm morphology | |||
| Morphologically normal (%) | 88.9 ± 0.6 | 89.3 ± 0.7a | 90.4 ± 0.6a |
| Abnormal head (%) | 1.9 ± 0.6 | 3.4 ± 0.4a | 2.3 ± 1.0a |
| Abnormal tail (%) | 5.9 ± 0.6 | 4.0 ± 0.7a | 4.2 ± 0.7a |
| Abnormal head and tail (%) | 3.3 ± 0.7 | 3.3 ± 0.5a | 3.1 ± 0.2a |
| Sperm flagella b | |||
| Morphologically normal (%) | 90.9 ± 0.5 | 92.7 ± 0.8a | 92.5 ± 0.6a |
| Absent (%) | 2.4 ± 0.4 | 1.9 ± 0.3a | 1.6 ± 0.3a |
| Short (%) | 1.1 ± 0.4 | 1.0 ± 0a | 1.1 ± 0.1a |
| Coiled (%) | 3.0 ± 0.9 | 2.4 ± 0.7a | 2.8 ± 0.6a |
| Bent (%) | 1.0 ± 0.3 | 1.0 ± 0.3a | 0.8 ± 0.1a |
| Irregular caliber (%) | 1.6 ± 0.1 | 1.0 ± 0.3a | 1.2 ± 0.3a |
For fertility test, three Dnah17+/+, three Dnah17+/M, and five Dnah17M/M male mice (10 wk old) were each caged with two WT females (C57BL/6J; 10 wk old) for 90 d. For semen analysis, three 10-wk-old mice were examined for each genotype. Data are presented as mean ± SEM. ***P < 0.001; compared with the Dnah17+/+ mice, one-way ANOVA with Dunnett‘s multiple comparison test.
a
NS, not significant.
b
Each spermatozoon was classified as only one type of flagellar morphology according to its major abnormality.