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. 2019 Dec 11;217(2):e20190783. doi: 10.1084/jem.20190783

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© 2019 Narasimhan et al.

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Figure S1. — Characterization of glial cell cultures in vitro. (A) ICC for oligodendrocytes (Olig-2, A2B5, Nogo-A, CNP, and MBP), astrocytes (GFAP), and microglia (Iba-1) in oligodendrocytes cultured from DIV4 to DIV26. Two independent experiments. Scale bar, 100 µm. (B) Double-labeling ICC for Olig-2, MBP, GFAP, or Iba-1 and tau (Tau5 or T49) in oligodendrocyte cultures. Two independent experiments. Scale bars, 100 µm; inset, 10 µm. (C) Quantification of oligodendrocytes, astrocytes, and microglia in oligodendrocyte culture over time. (D) IF quantification for T49 and Tau5 in oligodendrocyte cultures. (E) Western blot of T49, RD3 (3R tau), and RD4 (4R tau) for oligodendrocytes, astrocytes, and neurons in culture. β-tubulin is loading control. Three independent experiments. (F) ICC for GFAP, S100β, Olig-2, and Iba-1 in astrocyte cultures. Two independent experiments. Scale bar, 100 µm. (G) ICC for GFAP, T49, or Tau5 and DAPI in astrocyte cultures. Two independent experiments. Scale bar, 100 µm.