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. 2019 Nov 7;217(2):e20191520. doi: 10.1084/jem.20191520

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© 2019 Bando et al.

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Figure 1. — No evidence for IL-10–producing Lin⁻CD127⁺Thy1⁺ cells in naive small and large intestine. (A and B) Representative FACS plots showing IL-10–eGFP expression in small intestine (SI) and large intestine (LI) lamina propria (LP) CD45⁺ lymphocyte-sized Lin⁻CD45⁺CD127⁺ cells (A) and CD4⁺ T cells (B). (C) Frequencies of eGFP⁺ cells among lymphocyte-sized Lin⁻CD45⁺CD127⁺Thy1⁺ cells and CD4⁺ T cells in small intestine and large intestine lamina propria in multiple mice (n = 4). (D) Modified gating scheme to account for all cell lineages that expressed eGFP within the lymphocyte-based FSC/SSC gate. (E) Modified gating scheme to include cells with an expanded FSC/SSC profile. Below, the FSC/SSC profile of CD3e⁺ cells is shown as a reference for where lymphocytes lie in the plot. Bars indicate means ± SD. Data are representative of two independent experiments.