Figure S3.

Signaling events in Pggt1b^−/− thymocyte and homeostasis of Fntb^−/− mice. (A) Immunoblot analysis of p-Pak1/2 and Tiam1 in semimature CD4SP thymocytes from WT and Pggt1b^−/− mice upon CCL19 stimulation. (B) Immunoblot analysis of p-Mob1 in mature CD4SP thymocytes from WT and Pggt1b^−/− mice upon CCL19 stimulation. (C) Immunoblot analysis of p-Pak1/2 or p-Foxo1/3a in semimature CD4SP thymocytes from WT mice upon CCL19 or anti-CD3/28 stimulation. (D) Immunoblot analysis of Hdj-2 in mature CD4SP thymocytes from WT and Fntb^−/− mice. (E) Immunoblot analysis of p-Pak1/2 and Tiam1 in mature CD4SP thymocytes from WT and Fntb^−/− mice upon CCL19 stimulation. (F) Frequencies (left) and numbers (right) of CD4⁺ T cells and CD8⁺ T cells in PLN of WT and Fntb^−/− mice. (G) Frequencies of CD44^loCD62L^hi naive (left) and CD44^hiCD62L^lo effector/memory (right) cells in CD4⁺ or CD8⁺ T cells from PLNs of WT and Fntb^−/− mice. (H) Frequencies of Ki-67⁺ (left) and active caspase-3⁺ (right) cells in CD4⁺ or CD8⁺ T cells from PLNs of WT and Fntb^−/− mice. (I) Expression of CD44 and CD69, as indicated by MFI, on WT and Fntb^−/− naive CD4⁺ T cells upon overnight stimulation with plate-bound anti-CD3/28 (n = 9 per genotype). Data are shown as mean ± SEM. *, P < 0.05; **, P < 0.01; two-tailed unpaired Student’s t test in F–I. Data are from two (A–D) or four (F–H) independent experiments.