Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 22;217(2):e20191792. doi: 10.1084/jem.20191792

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Zhao et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Figure 5. — Variation at residue 204 in DII is responsible for the neutralization difference between two DENV1 strains. (A) Cartoon of DENV1 E protein (DI, red; DII, yellow; DIII, blue). ZIKV-116 epitope is highlighted in magenta. Amino acid differences (339 and 345) between 16007 and West Pac-74 (WP) DIII are shown in cyan. Residue 204, which is responsible for the difference in neutralization between two DENV1 strains, is marked as a black triangle in DII. (B and C) Comparison of K_D and t_1/2 of ZIKV-116 to DIII from H/PF/2013, 16007, and West Pac-74. The results from at least two independent experiments are shown. Error bars represent SD. (D–F) Effects of residue 204 on ZIKV-116 neutralization of DENV1 strains 16007 and West Pac-74. Neutralization profiles of mAbs against WT DENV1 strains 16007 and West Pac-74 RVPs compared with mutant RVPs containing reciprocal amino acid at residue 204 (upper). IC₅₀ values are from three independent experiments (bottom). DENV1-E111, which was reported to display differential neutralization against DENV1 genotypes, is used as a positive control mAb, and WNV-E60 serves as a negative control mAb. Statistical significance was determined by one-way ANOVA with a Tukey’s test (B and C) or two-tailed t tests (D–F). ns, not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001. FP, H/PF/2013.