Figure S3.

Time and temperature dependence of ZIKV inhibition. (A–C) ZIKV H/PF/2013 was incubated with ZIKV-116 or media for 1, 4, and 20 h at 4°C and 37°C (A and B) or 1 and 4 h at 40°C (C). Virus-mAbs or viruses alone were added to Vero cells after incubation, and infectivity was assessed 40 h later using an FFA assay. The relative infection was calculated by comparing no mAb-treated wells at each incubation time for each temperature. Data shown are pooled from two independent experiments in duplicate or triplicate. Error bars indicate SD.