Table S8. BLI results for ZIKV and DENV1 DIII binding to mAbs.

mAbs	DIII	Binding parametersa
		ka (105 M−1 s−1)	kd (10−3 s−1)	KD (nM), kinetic	KD (nM), equilibriumb		t1/2 (min)c
ZIKV-116	DIIIH/PF/2013 (pH 5.5)	3.27 ± 0.29	0.73 ± 0.13	2.24 ± 0.19	5.05 ± 0.41	16.06 ± 2.62
ZIKV-116	DIIIH/PF/2013	3.94 ± 0.14	0.45 ± 0.03	1.15 ± 0.07	7.54 ±1.56	25.7 ± 1.84
ZIKV-116	DIIIMR-766	3.91 ± 0.26	47.17 ± 6.40	121 ± 16.52	141 ± 22.34	0.25 ± 0.03
ZIKV-116	DIIIH/PF/2013 (E393D)	3.76 ± 0.52	22.83 ± 5.28	62 ± 19.93	68.67 ± 9.32	0.52 ± 0.12
ZIKV-116	DIII16007	1.84 ± 0.54	1.90 ± 0.08	10.7 ± 2.70	15.95 ± 6.72	6.10 ± 0.25
ZIKV-116	DIIIWest Pac-74	1.47 ± 0.41	1.86 ± 0.01	13.2 ± 3.82	16.75 ± 3.89	6.21 ± 0.04
ZIKV-116 V.J.Rev	DIIIH/PF/2013	2.02 ± 0.20	9.82 ± 0.10	48.9 ± 5.37	68.15 ± 17.18	1.18 ± 0.01
ZIKV-116 V.J.Rev	DIIIMR-766	0.65 ± 0.18	790.7 ± 194.6	12,233 ± 986.6	16,267 ± 2,479	0.015 ± 0.004
ZIKV-116 V.J.Rev	DIII16007	0.34 ± 0.12	229.0 ± 9.90	7,160 ± 2,220	39,050 ± 34,578	0.05 ± 0.002
ZIKV-116 V.J.Rev	DIIIWest Pac-74	0.31 ± 0.09	199.5 ± 48.79	6,955 ± 3557	48,300 ± 41,861	0.065 ± 0.01

^aValues are means ± SD from at least two independent experiments.

^bK_D, equilibrium was determined as described in the Materials and methods.

^cCalculated from the dissociation constant: t_1/2 = ln(2)/k_d.