Table S9. Key resources.
Reagent or resource | Source | Identifier |
---|---|---|
Antibodies | ||
Monoclonal anti-ZIKV-E ZIKV-116 | This paper | N/A |
Monoclonal anti-WNV-E E60 | Michael S. Diamond, WUSTL, (mdiamond@wustl.edu) | Oliphant et al., 2006 |
Monoclonal anti-WNV-E E53 | Michael S. Diamond, WUSTL | Oliphant et al., 2006 |
Monoclonal anti-WNV WNV-E111 | Michael S. Diamond, WUSTL | Oliphant et al., 2006 |
Monoclonal anti-DENV1 DENV1-E111 | Michael S. Diamond, WUSTL | Austin et al., 2012 |
Monoclonal anti-DENV4 DENV4-E88 | Michael S. Diamond, WUSTL | Sukupolvi-Petty et al., 2013 |
Monoclonal anti-JEV JEV-106 | Michael S. Diamond, WUSTL | Fernandez et al., 2018 |
Monoclonal anti-POWV POWV-61 | This paper, unpublished mAb | N/A |
Goat anti-human IgG-HRP | Santa Cruz Biotechnology | Cat# sc-2907 |
Goat anti-mouse IgG-HRP | Santa Cruz Biotechnology | Cat# sc-2005 |
Goat anti-mouse-PE | BD Pharmingen | Cat# 550589 |
Bacterial and virus strains | ||
ZIKV strain: French Polynesia_H/PF/2013 | Michael S. Diamond, WUSTL | Zhao et al., 2016 |
ZIKV strain: Uganda_MR-766 | Michael S. Diamond, WUSTL | Zhao et al., 2016 |
ZIKV strain: Senegal_Dakar 41519 | Michael S. Diamond, WUSTL | Zhao et al., 2016 |
DENV1 strain 16007 | Michael S. Diamond, WUSTL | Shrestha et al., 2010 |
DENV1 strain West Pac-74 | Michael S. Diamond, WUSTL | Shrestha et al., 2010 |
BL21-CodonPlus-RIL competent cells | Agilent Technologies | Cat# 230240 |
Chemicals, peptides, and recombinant proteins | ||
EZ-Link-NHS-PEG4-Biotin | Thermo Fisher | Cat# 21330 |
Recombinant ZIKV-DIII from H/PF/2013 | This paper | GenBank: AHZ13508.1 |
Recombinant ZIKV-DIII from MR-766 | This paper | GenBank: ANO46296.1 |
Recombinant DENV1-DIII from 16007 | Austin et al., 2012 | N/A |
Recombinant DENV1-DIII from West Pac-74 | Austin et al., 2012 | N/A |
Recombinant DENV2-DIII | Austin et al., 2012 | N/A |
Recombinant DENV3-DIII | Austin et al., 2012 | N/A |
Recombinant DENV4-DIII | Austin et al., 2012 | N/A |
Recombinant WNV-DIII from New York 1999 | Nybakken et al., 2005 | N/A |
Recombinant WNV-E from New York 1999 | Luca et al., 2012 | N/A |
Recombinant JEV-DIII from SA14-14-2 | Fernandez et al., 2018 | N/A |
Recombinant SPOV-E from SM6 V-1 | This paper | GenBank: YP_009227187.1 |
Recombinant POWV-DIII from Spooner | This paper | GenBank: ADK37756.1 |
Critical commercial assays | ||
RNEasy Mini Purification Kit | Qiagen | Cat# 74104 |
SuperScript III First-Strand Synthesis System Thermo Fisher for RT-PCR | Thermo Fisher | Cat# 18080-051 |
4–12% Bis-Tris NuPAGE gel system | Thermo Fisher | Cat# 18080-051 |
FuGENE 6 Transfection Reagent | Cat# E2691 | |
Deposited data | ||
ZIKV-116 Fab in complex with DIII structure | This paper | PDB: 6PLK |
Experimental models: cell lines | ||
Vero | ATCC | Cat# CCL-81 |
Human: FreeStyle 293F | Thermo Fisher | Cat# R79007 |
Human: FreeStyle 293F | Thermo Fisher | Cat# R79007 |
HEK 293T | ATCC | Cat# CRL-1573 |
Raji-DCSIGNR | Pierson laboratory | N/A |
Recombinant DNA | ||
Plasmid: pML-huCG1 | This paper | N/A |
Plasmid: pET21a | This paper | N/A |
Software and algorithms | ||
GraphPad Prism | GraphPad Software, Inc. | https://www.graphpad.com |
FlowJo version 10 | Tree Star | https://www.flowjo.com/solutions/flowjo/downloads |
Biaevaluation 4.1 | GE Healthcare | https://www.biacore.com/lifesciences/service/downloads/software_licenses/biaevaluation/ |
Phaser | McCoy et al., 2007 | http://www.ccp4.ac.uk/html/phaser.html |
Phenix | Adams et al., 2010 | https://www.phenix-online.org/ |
Coot | Emsley et al., 2010 | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
PDBePISA | Open source | www.ebi.ac.uk/pdbe/pisa/ |
PyMOL | Schrödinger | https://www.pymol.org/ |
Ligplot | McDonald and Thornton, 1994 | https://sbgrid.org/software/titles/ligplot |
Agilent QuikChange Primer Design program | Agilent Technologies | N/A |
N/A, not applicable; WUSTL, Washington University in St. Louis.