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Knockdown of P3H4 arrested cell cycle in the G1 phase and thus inhibited proliferation, but had no effect on apoptosis. After siP3H4 was transfected into EJ and T24 cells, (A) the clonality was evaluated by clone formation assay, (B and C) cell cycle was detected by flow cytometry, (D) the protein expression of cell cycle-related proteins Cyclin D1, Nusap1 and p70 were measured by western blot, (E) apoptosis was examined by flow cytometry. *P<0.05.
