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. 2020 Feb 4;12(3):2440–2452. doi: 10.18632/aging.102753

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Copyright © 2020 Zou et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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HO-1 overexpression induces autophagy in human NPCs. (A) Western bolt shows HO-1, P62 and LC3-II/I protein levels in control and HO-1 overexpressing NPCs. HO-1 overexpressing NPCs were generated by transfecting Ad-HO-1 for 48 h. Note: The data represent mean ± SD of three experiments; **p<0.01. (B) Immunofluorescence assay results show autophagosome formation based on staining of the control and HO-1 overexpressing NPCs with antibodies against the autophagosome marker protein SQSTM1/P62. (C) Transmission electron micrographs show characteristic double-membrane autophagosome formation (black arrows) in control and HO-1 overexpressing NPCs. (D) Western bolt shows HO-1, P62 and LC3-II/I protein levels in control and HO-1 siRNA transfected NPCs. As shown, there is no significant difference in the levels of these proteins in all experimental groups. Note: The data represent mean ± SD of three experiments.