Fig 1. Tick saliva proteins composition in Amblyomma americanum tick saliva every 24 h during feeding.

Pilocarpine induced A. americanum tick saliva was subjected to LC-MS/MS sequencing using the “In-Solution” digestion approach as described in materials and methods. Cumulative normalized spectral abundance factors (NSAF) values as index for relative abundance of detected tick saliva proteins are presented for each functional class. Please note the labels are oriented horizontally and read left to right starting from cytoskeletal proteins (red), antioxidant/detoxification (green), extracellular matrix (orange), then back to glycine rich proteins (light-tan), immune related (pink) and continues this pattern.