Table 3. Numbers and cumulative relative abundance of rabbit protein classes in Amblyomma americanum saliva during 24–120 h of feeding (NSAF = normalized spectral abundance factor).
| Feeding Time Point | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 24 h | 48 h | 72 h | 96 h | 120 h | ||||||
| Classification | Protein Count | NSAF (%) | Protein Count | NSAF (%) | Protein Count | NSAF (%) | Protein Count | NSAF (%) | Protein Count | NSAF (%) |
| antimicrobial | 3 | 0.00134357 | 4 | 0.00192864 | 3 | 0.00171397 | 5 | 0.0086771 | 6 | 0.00763598 |
| antioxidant | 2 | 0.00042155 | 1 | 7.576E-05 | 1 | 0.00035322 | 0 | 0 | 0 | 0 |
| cytoskeletal | 19 | 0.01798265 | 14 | 0.01629188 | 11 | 0.00953933 | 12 | 0.01698913 | 7 | 0.01026072 |
| extracellular matrix | 3 | 0.00049492 | 1 | 0.000267 | 1 | 0.0000291 | 2 | 0.0001466 | 2 | 0.0003605 |
| fibrinogen | 3 | 0.00022576 | 4 | 0.00017475 | 0 | 0 | 1 | 6.7612E-05 | 2 | 0.00022745 |
| globin/ RBC | 20 | 0.08991802 | 18 | 0.02390767 | 12 | 0.01107268 | 15 | 0.02658361 | 15 | 0.02722383 |
| heme/Iron binding | 5 | 0.01948816 | 7 | 0.01314713 | 2 | 0.00443612 | 7 | 0.00916951 | 4 | 0.00596773 |
| immunity related | 9 | 0.00268497 | 7 | 0.0023492 | 5 | 0.00211006 | 8 | 0.00712898 | 15 | 0.01097912 |
| keratin | 17 | 0.00337019 | 32 | 0.01031924 | 35 | 0.02503904 | 41 | 0.04692297 | 27 | 0.03080678 |
| lipocalin | 0 | 0 | 1 | 0.00051272 | 1 | 0.00052663 | 1 | 0.00283525 | 1 | 0.00167895 |
| metabolism, amino acid | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| metabolism, carbohydrates | 5 | 0.00133936 | 4 | 0.00037672 | 5 | 0.00064097 | 5 | 0.00074812 | 3 | 0.00033307 |
| metabolism, energy | 1 | 2.8207E-05 | 0 | 0 | 0 | 0 | 3 | 0.00071594 | 1 | 0.00018482 |
| metabolism, lipid | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0.00093604 | 2 | 0.00138574 |
| metabolism, nucleic acids | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| nuclear regulation | 8 | 0.00325694 | 16 | 0.00871704 | 15 | 0.01681692 | 18 | 0.01938734 | 15 | 0.01609353 |
| protease | 0 | 0 | 1 | 0.00013596 | 0 | 0 | 2 | 0.00146001 | 1 | 4.2416E-05 |
| protease Inhibitors | 8 | 0.0025413 | 5 | 0.00116042 | 1 | 0.0001394 | 5 | 0.00178596 | 1 | 0.00071414 |
| protein export | 0 | 0 | 2 | 0.00017604 | 2 | 0.00115523 | 3 | 0.00209215 | 3 | 0.00247782 |
| protein modification | 7 | 0.00102993 | 3 | 0.0003923 | 7 | 0.00221869 | 6 | 0.00095378 | 4 | 0.00051132 |
| protein synthesis | 4 | 0.00135166 | 2 | 0.0001756 | 4 | 0.00122153 | 4 | 0.0006344 | 0 | 0 |
| proteasome machinery | 2 | 0.00600273 | 2 | 0.0021349 | 2 | 0.00578655 | 2 | 0.0056957 | 2 | 0.00490593 |
| signal transduction | 9 | 0.00264391 | 1 | 0.00047693 | 7 | 0.00264375 | 1 | 0.00089234 | 1 | 0.00035228 |
| transporter/ receptor | 2 | 0.00013431 | 5 | 0.00049163 | 1 | 3.5648E-05 | 4 | 0.00061835 | 0 | 0 |
| transcription machinery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Total | 127 | 0.15425811 | 130 | 0.08321153 | 115 | 0.08547884 | 147 | 0.15444091 | 112 | 0.12214214 |