Table 4. Numbers and cumulative relative abundance of rabbit protein classes in Amblyomma americanum saliva during 144 to completion of feeding (NSAF = normalized spectral abundance factor).
| Feeding Time Point | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 144 h | 168 h | 192 h | BD | SD | ||||||
| Classification | Protein Count | NSAF (%) | Protein Count | NSAF (%) | Protein Count | NSAF (%) | Protein Count | NSAF (%) | Protein Count | NSAF (%) |
| antimicrobial | 8 | 0.007565 | 9 | 0.018337 | 9 | 0.015212 | 6 | 0.004394 | 8 | 0.015088 |
| antioxidant | 3 | 0.000677 | 2 | 0.00071 | 3 | 0.000384 | 0 | 0 | 4 | 0.000927 |
| cytoskeletal | 20 | 0.019936 | 29 | 0.014844 | 26 | 0.019163 | 6 | 0.011922 | 55 | 0.031601 |
| extracellular matrix | 1 | 3.63E-05 | 0 | 0 | 1 | 2.58E-05 | 1 | 8.41E-05 | 3 | 0.000973 |
| fibrinogen | 1 | 0.00011 | 5 | 0.002512 | 5 | 0.001638 | 0 | 0 | 5 | 0.001423 |
| globin/ RBC | 18 | 0.105112 | 19 | 0.100601 | 18 | 0.067832 | 16 | 0.052725 | 21 | 0.109247 |
| heme/Iron binding | 3 | 0.005258 | 9 | 0.011255 | 7 | 0.011786 | 3 | 0.005854 | 9 | 0.015765 |
| immunity related | 15 | 0.007953 | 23 | 0.02019 | 17 | 0.014264 | 5 | 0.002246 | 23 | 0.025243 |
| keratin | 20 | 0.010315 | 34 | 0.012893 | 37 | 0.021782 | 21 | 0.010099 | 40 | 0.045599 |
| lipocalin | 1 | 0.002122 | 1 | 0.004206 | 1 | 0.00311 | 1 | 0.001514 | 1 | 0.00534 |
| metabolism, amino acid | 0 | 0 | 1 | 0.000201 | 0 | 0 | 0 | 0 | 1 | 3.83E-05 |
| metabolism, carbohydrates | 5 | 0.001337 | 9 | 0.001486 | 7 | 0.001096 | 2 | 0.000114 | 7 | 0.001634 |
| metabolism, energy | 2 | 0.000319 | 2 | 0.000144 | 2 | 0.000364 | 0 | 0 | 6 | 0.000535 |
| metabolism, lipid | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0.000229 |
| metabolism, nucleic acids | 1 | 0.000108 | 4 | 0.000989 | 0 | 0 | 0 | 0 | 0 | 0 |
| nuclear regulation | 19 | 0.013472 | 20 | 0.028935 | 24 | 0.022492 | 10 | 0.008565 | 26 | 0.032846 |
| protease | 0 | 0 | 4 | 0.000876 | 1 | 9.16E-05 | 0 | 0 | 6 | 0.00066 |
| protease Inhibitors | 1 | 0.000202 | 3 | 0.000967 | 2 | 0.000151 | 0 | 0 | 7 | 0.00203 |
| protein export | 3 | 0.001588 | 4 | 0.004882 | 5 | 0.003771 | 2 | 0.00077 | 4 | 0.006886 |
| protein modification | 6 | 0.000988 | 7 | 0.000754 | 12 | 0.001567 | 0 | 0 | 16 | 0.002522 |
| protein synthesis | 0 | 0 | 4 | 0.00057 | 4 | 0.00084 | 0 | 0 | 4 | 0.000866 |
| proteasome machinery | 2 | 0.003209 | 2 | 0.003649 | 2 | 0.003418 | 2 | 0.000584 | 2 | 0.005294 |
| signal transduction | 8 | 0.003392 | 5 | 0.001237 | 9 | 0.001945 | 0 | 0 | 12 | 0.003857 |
| transporter/ receptor | 3 | 0.000381 | 1 | 6.81E-05 | 4 | 0.000521 | 3 | 0.000589 | 13 | 0.00182 |
| transcription machinery | 0 | 0 | 2 | 6.16E-05 | 2 | 6.68E-05 | 0 | 0 | 7 | 0.000636 |
| Total | 140 | 0.184079 | 199 | 0.230369 | 198 | 0.191521 | 78 | 0.099459 | 282 | 0.311061 |