Table 1. Mean and Standard Error Values for the Apparent Enthalpy and Kinetics Parameters of hsEH CTD-Mediated Hydrolysis of the EpFAs Analyzed in This Study^a.

substrate	ΔHapp [kJ mol–1]	kcat [s–1]	KM [μM]	Ksp [s–1 μM–1]
5(6)EET	–4.84 ± 0.65	0.35 ± 0.05	46.61 ± 10.98	0.01 ± 0.001
8(9)EET	–39.45 ± 3.88	1.28 ± 0.19	23.10 ± 2.39	0.054 ± 0.010
11(12)EET	–12.43 ± 0.62	4.31 ± 0.16	1.74 ± 0.20	2.53 ± 0.25
14(15)EET	–23.72 ± 3.46	6.64 ± 1.54	12.88 ± 1.94	0.527 ± 0.075
8(9)EpETE	–34.13 ± 0.93	1.61 ± 0.01	9.74 ± 0.13	0.173 ± 0.006
17(18)EpETE	–51.78 ± 1.73	0.64 ± 0.06	26.37 ± 4.05	0.025 ± 0.003
19(20)EpDPE	–23.73 ± 1.82	1.62 ± 0.08	26.60 ± 1.21	0.061 ± 0.003
12(13)EpOME	–5.67 ± 0.38	9.65 ± 0.19	2.98 ± 0.56	3.56 ± 0.86

^aThe values were obtained as described in the methods.