Table 3.
Cerebellum total phospholipid fatty acid concentration and composition at 2 months old and >7 months old (mean ± SD; 2 month-old n = 5/group, >7 month-old, n = 4-7/group).
| FA | Wildtype (Rora+/+) | Staggerer (Rorasg/sg) | Wildtype (Rora+/+) | Staggerer (Rorasg/sg) |
|---|---|---|---|---|
| nmol/g brain (mol%) | nmol/g brain (mol%) | nmol/g brain (mol%) | nmol/g brain (mol%) | |
| 2 month-old | 2 month-old | >7 month-old | >7 month-old | |
| 14:0 | 241 (0.2) ± 69 (0.05) | 98 (0.3) ± 43** (0.07) | 225 (0.2) ± 174 (0.1) | 170 (0.2) ± 111 (0.01) |
| 16:0 | 28054 (25) ± 2584 (0.6) | 7111 (22) ± 1565*** (1.4##) | 29390 (25) ± 8064 (1.5) | 26649 (26) ± 14357 (11) |
| 18:0 | 23744 (21) ± 2270 (0.4) | 6320 (20) ± 1110*** (0.7##) | 23420 (20) ± 6459 (0.9) | 26847 (27) ± 14482 (14) |
| 20:0 | 520 (0.5) ± 29 (0.05) | 266 (0.8) ± 73*** (0.1##) | 440 (0.4) ± 119 (0.1) | 1049 (1.0) ± 607 (0.6) |
| 22:0 | 354 (0.3) ± 35 (0.06) | 196 (0.6) ± 42*** (0.1##) | 343 (0.3) ± 74 (0.07) | 841 (0.8) ± 486 (0.5) |
| 24:0 | 494 (0.4) ± 69 (0.1) | 271 (0.9) ± 58*** (0.2##) | 426 (0.4) ± 165 (0.1) | 977 (1.0) ± 581 (0.5) |
| 16:1n-7 | 617 (0.5) ± 78 (0.07) | 246 (0.8) ± 64*** (0.1#) | 689 (0.6) ± 152 (0.07) | 617 (0.5) ± 616 (0.3) |
| 18:1n-9 | 22636 (20) ± 2669 (0.6) | 8339 (26) ± 1585*** (1.7###) | 23924 (21) ± 4551 (2.2) | 24894 (20) ± 24692 (13) |
| 18:1n-7 | 5989 (5.3) ± 618 (0.09) | 1904 (5.9) ± 411*** (0.5) | 5796 (5.1) ± 1073 (0.6) | 4941 (4.0) ± 4814 (2.5) |
| 20:1n-9 | 3145 (2.8) ± 423 (0.09) | 1050 (3.3) ± 333*** (0.9) | 3483 (3.1) ± 973 (0.9) | 4449 (3.5) ± 4459 (2.2) |
| 22:1n-9 | 239 (0.2) ± 11 (0.02) | 102 (0.3) ± 29*** (0.09#) | 194 (0.2) ± 53 (0.04) | 309 (0.3) ± 323 (0.2) |
| 24:1n-9 | 839 (0.7) ± 71 (0.1) | 410 (1.3) ± 122*** (0.4#) | 1258 (1.1) ± 374 (0.3) | 2255 (1.8) ± 1886 (0.4#) |
| 18:2n-6 | 905 (0.8) ± 87 (0.06) | 505 (1.6) ± 632 (2.0) | 773 (0.7) ± 148 (0.06) | 398 (0.4) ± 376 (0.2) |
| 18:3n-6 | 0 (0) ± 0 (0) | 0 (0) ± 0 (0) | 0 (0) ± 0 (0) | 0 (0) ± 0 (0) |
| 20:2n-6 | 309 (0.3) ± 22 (0.03) | 77 (0.2) ± 30*** (0.06) | 181 (0.2) ± 34 (0.03) | 189 (0.1) ± 214 (0.1) |
| 20:3n-6 | 516 (0.5) ± 37 (0.03) | 183 (0.6) ± 47*** (0.06#) | 450 (0.4) ± 93 (0.02) | 352 (0.3) ± 344 (0.2) |
| 20:4n-6 | 7000 (6.1) ± 1024 (0.3) | 2193 (6.7) ± 652*** (1.3) | 7552 (6.5) ± 2515 (0.8) | 5791 (4.8) ± 5565 (3.1) |
| 22:4n-6 | 1384 91.2) ± 234 (0.08) | 606 (1.9) ± 197*** (0.4#) | 1791 (1.5) ± 691 (0.3) | 2088 (1.7) ± 2006 (1.1) |
| 22:5n-6 | 82 (0.07) ± 23 (0.03) | 32 (0.1) ± 28* (0.09) | 75 (0.06) ± 75 (0.05) | 61 (0.06) ± 56 (0.04) |
| 18:3n-3 | 73 (0.06) ± 29 (0.02) | 29 (0.09) ± 5* (0.02) | 68 (0.06) ± 18 (0.01) | 115 (0.1) ± 74 (0.03) |
| 20:5n-3 | 79 (0.07) ± 10 (0.004) | 46 (0.1) ± 23* (0.07) | 62 (0.05) ± 15 (0.01) | 56 (0.07) ± 48 (0.07) |
| 22:5n-3 | 221 (0.2) ± 22 (0.02) | 97 (0.3) ± 20*** (0.04###) | 236 (0.2) ± 71 (0.01) | 233 (0.2) ± 253 (0.1) |
| 22:6n-3 | 16063 (14) ± 2772 (1.1) | 2252 (6.88) ± 732*** (1.5###) | 14393 (12) ± 4675 (1.5) | 7607 (6.1) ± 7324 (3.6#) |
| Total FA | 113503 ± 12518 | 32332 ± 6287*** | 115166 ± 27116 | 110888 ± 74438 |
Statistical significance for fatty acid concentration:
*
p < 0.05
**
p < 0.01
***
p < 0.001.
Statistical significance for fatty acid composition:
#
p < 0.05
##
p < 0.01
###
p < 0.001.
N denotes number of animals.