Table 5.
Prefrontal cortex total phospholipid fatty acid concentration and composition at 2 months old and >7 months old (mean ± SD; 2 month-old n = 4- 5/group, >7 month-old, n = 4-5/group).
| FA | Wildtype (Rora+/+) | Staggerer (Rorasg/sg) | Wildtype (Rora+/+) | Staggerer (Rorasg/sg) |
|---|---|---|---|---|
| nmol/g brain (mol%) | nmol/g brain (mol%) | nmol/g brain (mol%) | nmol/g brain (mol%) | |
| 2 month-old | 2 month-old | >7 month-old | >7 month-old | |
| 14:0 | 234 (0.2) ± 20 (0.01) | 230 (0.2) ± 29 (0.02) | 138 (0.1) ± 46 (0.03) | 126 (0.1) ± 17 (0.02) |
| 16:0 | 37034 (27) ± 1677 (0.5) | 36268 (28) ± 1666 (0.4#) | 34753 (27) ± 2010 (0.5) | 34235 (29) ± 1047 (0.5##) |
| 18:0 | 29586 (22) ± 1337 (0.2) | 27736 (21) ± 1568 (0.07) | 28019 (22) ± 2411 (0.6) | 25208 (21) ± 1395 (0.2) |
| 20:0 | 302 (0.2) ± 46 (0.03) | 233 (0.2) ± 40 (0.02) | 246 (0.2) ± 38 (0.03) | 196 (0.2) ± 37 (0.02) |
| 22:0 | 417 (0.3) ± 69 (0.04) | 294 (0.2) ± 100 (0.07) | 220 (0.2) ± 123 (0.08) | 140 (0.1) ± 13 (0.01) |
| 24:0 | 385 (0.3) ± 76 (0.05) | 277 (0.2) ± 118 (0.09) | 181 (0.1) ± 76 (0.05) | 137 (0.1) ± 36 (0.02) |
| 16:1n-7 | 772 (0.6) ± 37 (0.02) | 793 (0.6) ± 91 (0.05) | 739 (0.6) ± 101 (0.05) | 722 (0.6) ± 86 (0.06) |
| 18:1n-9 | 21180 (15) ± 1347 (0.3) | 19273 (15) ± 1055 (0.2#) | 22194 (17) ± 1327 (0.4) | 19837 (17) ± 1018* (0.1#) |
| 18:1n-7 | 5094 (3.7) ± 192 (0.06) | 4834 (3.7) ± 190 (0.1) | 5091 (3.9) ± 267 (0.1) | 4796 (4) ± 157 (0.06) |
| 20:1n-9 | 1381 (1) ± 124 (0.05) | 933 (0.7) ± 124** (0.08###) | 1691 (1.3) ± 187 (0.09) | 1240 (1) ± 165** (0.09##) |
| 22:1n-9 | 127 (0.09) ± 16 (0.01) | 88 (0.07) ± 16** (0.01##) | 109 (0.08) ± 15 (0.01) | 106 (0.09) ± 30 (0.02) |
| 24:1n-9 | 728 (0.5) ± 107 (0.06) | 417 (0.3) ± 152** (0.1##) | 756 (0.6) ± 230 (0.1) | 515 (0.4) ± 95 (0.06) |
| 18:2n-6 | 822 (0.6) ± 53 (0.06) | 826 (0.6) ± 59 (0.03) | 663 (0.5) ± 51 (0.03) | 571 (0.5) ± 22* (0.01) |
| 18:3n-6 | 0 (0) ± 0 (0) | 0 (0) ± 0 (0) | 0 (0) ± 0 (0) | 0 (0) ± 0 (0) |
| 20:2n-6 | 201 (0.1) ± 6 (0.01) | 181 (0.1) ± 7** (0.01) | 232 (0.2) ± 40 (0.04) | 265 (0.2) ± 35 (0.02) |
| 20:3n-6 | 580 (0.4) ± 68 (0.04) | 553 (0.4) ± 70 (0.03) | 472 (0.4) ± 49 (0.02) | 451 (0.4) ± 26 (0.01) |
| 20:4n-6 | 12881 (9.4) ± 677 (0.2) | 12179 (9.4) ± 724 (0.2) | 11754 (9.1) ± 773 (0.3) | 10468 (8.8) ± 467* (0.1) |
| 22:4n-6 | 3111 (2.3) ± 273 (0.1) | 2838 (2.2) ± 204 (0.09) | 3109 (2.4) ± 219 (0.1) | 2656 (2.2) ± 153** (0.03#) |
| 22:5n-6 | 266 (0.2) ± 17 (0.02) | 272 (0.2) ± 24 (0.02) | 204 (0.2) ± 31 (0.02) | 186 (0.2) ± 18 (0.01) |
| 18:3n-3 | 56 (0.04) ± 6 (0.01) | 44 (0.03) ± 15 (0.01) | 30 (0.02) ± 11 (0.01) | 28 (0.02) ± 8 (0.01) |
| 20:5n-3 | 53 (0.04) ± 14 (0.01) | 51 (0.04) ± 8 (0.01) | 50 (0.04) ± 2 (0.003) | 52 (0.04) ± 9 (0.01) |
| 22:5n-3 | 248 (0.2) ± 27 (0.01) | 262 (0.2) ± 23 (0.01) | 241 (0.2) ± 42 (0.02) | 222 (0.2) ± 12 (0.01) |
| 22:6n-3 | 21371 (16) ± 772 (0.4) | 20704 (16) ± 1523 (0.3) | 18460 (14) ± 1431 (0.5) | 17232 (14) ± 876 (0.4) |
| Total FA | 136829 ± 6392 | 129286 ± 7450 | 129353 ± 8609 | 119390 ± 5422 |
Statistical significance for fatty acid concentration:
*
p < 0.05
**
p < 0.01
***
p < 0.001.
Statistical significance for fatty acid composition:
#
p < 0.05
##
p < 0.01
###
p < 0.001.
N denotes number of animals.