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. 2020 Feb 18;11(7):687–698. doi: 10.18632/oncotarget.27479

Figure 5. Effects of Mifamurtide (MTP) on TNF-α, MMP2, MMP9 and TRPV1 expression levels in MG63 alone and co-cultured with macrophages.

Figure 5

(A) TNF-α mRNA expression levels in MG63 alone or in co-culture with macrophages activated with MTP [100 µM] or not determined by RT-qPCR. (B, C) MMP2/MMP9 and TRPV1 mRNA expression levels in MG63 co-cultured with macrophages activated or not with MTP [100 µM] determined by RT-qPCR. Results were normalized for the housekeeping gene β-Actin and showed as mean ± SD of three independent experiments. A t-test has been used to evaluate statistical differences in mRNA expression levels. *indicates p ≤ 0.05 compared to macrophages-MG63 co-culture, ^ indicates p ≤ 0.05 compared to MG63 alone.