Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (human, female) | HEK293 | ATCC | CRL-1573; RRID: CVCL_0045 | Human embryonic kidney |
| Antibody | Mouse anti-FLAG (M1) | Sigma-Aldrich | F-3040; RRID: AB_439712 | (1:1000) |
| Antibody | Rabbit anti-GFP | Invitrogen | A-11122; RRID: AB_221569 | (1:100) |
| Recombinant DNA reagent | EGFP-Nb33 | (Stoeber et al., 2018) | N/A | EGFP-C1 backbone |
| Recombinant DNA reagent | pmApple-Nb33 | (Stoeber et al., 2018) | N/A | pmApple-C1 backbone |
| Recombinant DNA reagent | NES-Venus-mGsi | (Wan et al., 2018) | N/A | pcDNA3 backbone |
| Recombinant DNA reagent | NES-Venus-mGs | (Wan et al., 2018) | N/A | pcDNA3 backbone |
| Recombinant DNA reagent | signal sequence FLAG (ssf)-MOR, murine | (Stoeber et al., 2018) | N/A | pcDNA3 backbone |
| Recombinant DNA reagent | ssf-KOR, murine | (Chu et al., 1997) | N/A | pcDNA3 backbone |
| Recombinant DNA reagent | ssf-KOR-TPD (S356A, T357A, T363A, S369A) | This study | N/A | pcDNA3 backbone, see Materials and methods |
| Recombinant DNA reagent | Super ecliptic pHluorin (SEP) -KOR, murine | This study | N/A | pCAGGS-SE backbone, see Materials and methods |
| Recombinant DNA reagent |
GRK2-EGFP, murine | This study | N/A | pCAGGS-SE backbone, see Materials and methods |
| Recombinant DNA reagent | GRK2-pmApple, murine | This study | N/A | pCAGGS-SE backbone, see Materials and methods |
| Recombinant DNA reagent | GRK2-C-tail- EGFP (aa 546–670) | This study | N/A | pCAGGS-SE backbone, see Materials and methods |
| Peptide, recombinant protein | Dynorphin A (1–17, DynA) | Anaspec | AS-24298 | |
| Peptide, recombinant protein | DAMGO, [D-Ala2, N-Me-Phe4, Gly5-ol]-Enkephalin acetate salt | Sigma-Aldrich | E7384 | |
| Chemical compound, drug | U-69593 (U69) | Cayman Chemical | 13255 | |
| Chemical compound, drug | U-50488 hydrochloride (U50) | Tocris | 0495 | |
| Chemical compound, drug | GNTI dihydrochloride (5’GNTI) | Axon Med Chem | 1226 | |
| Chemical compound, drug | Etorphine-HCl | NIDA | N/A | |
| Chemical compound, drug | Morphine sulfate (MS) | Sigma-Aldrich | 1448005 | |
| Chemical compound, drug | Naloxone hydrochloride dihydrate | Sigma-Aldrich | N7758 | |
| Chemical compound, drug | PZM21 | Enamine | N/A | custom synthesis |
| Chemical compound, drug | mitragynine pseudoindoxyl (MP) | This study | N/A | (Váradi et al., 2016) |
| Chemical compound, drug | Compound101 (Cmpd101) | HelloBio | HB2840 | |
| Chemical compound, drug | Pertussis toxin | Sigma-Aldrich | P7208 | |
| Commercial assay or kit | Alexa Fluor 647 Protein Labeling Kit | Thermo Fisher Scientific | A20173 | |
| Software, algorithm | Prism | GraphPad | 8.1.1 | |
| Software, algorithm | ImageJ | Imagej.net/contributors | 2.0.0-rc-54/1.51 g | |
| Software, algorithm | MATLAB | MathWorks | R2014b | |
| Software, algorithm | PyMOL | Schrödinger | v1.7.4.5 |