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. 2020 Feb 25;9:e49917. doi: 10.7554/eLife.49917

Figure 2. daf-2b splicing and expression are subject to temporal and spatial regulation.

(A) Schematic illustrating the organization of the daf-2a/c splicing reporter. (B) Schematic illustrating the organization of the daf-2b splicing reporter. (C) Representative fluorescence images showing the expression of DAF-2A/C::tdTomato through reproductive development and in dauers in animals bearing extrachromosomal arrays. All images were taken with 1 s exposure. (D) Representative fluorescence images showing the expression of DAF-2B::tdTomato through reproductive development and in dauers in animals bearing an integrated array. All images were taken with 1.5 s exposure. (E) Schematic illustrating the location of mScarlet insertion into the daf-2 genomic locus. Neuronal expression of DAF-2B::mScarlet in L1 (F), young adults (G) and dauers (H). Accumulation of DAF-2B::mScarlet in coelomocytes in L1 (I), young adults (J) and dauer (K) animals. DAF-2B::tdTomato from the transgenic splicing reporter is not expressed in coelomocytes in L1 (L), young adults (M) or dauers (N). Arrows indicate neuronal expression.

Figure 2.

Figure 2—figure supplement 1. Tissue localization of the DAF-2A/C::tdTomato splicing reporter.

Figure 2—figure supplement 1.

(A) Expression of DAF-2A/C::tdTomato with ges-1p::GFP in the intestine in an L1 animal. (B) Expression of DAF-2A/C::tdTomato with dpy-7p::GFP in the hypodermis in an L1 animal. (C) Expression of DAF-2A/C::tdTomato with rgef-1p::GFP in neurons in a young adult animal. (D) Expression of DAF-2A/C::tdTomato with ges-1p::GFP in the intestine in a dauer. (E) Expression of DAF-2A/C::tdTomato with rgef-1p::GFP in neurons in a dauer.
Figure 2—figure supplement 2. Tissue localization of the DAF-2B::tdTomato splicing reporter.

Figure 2—figure supplement 2.

(A) Expression of DAF-2B::tdTomato with ges-1p::GFP in the intestine in an L1 animal. (B) Expression of DAF-2B::tdTomato with dpy-7p::GFP in the hypodermis in an L1 animal. (C) Expression of DAF-2B::tdTomato with rgef-1p::GFP in neurons in a young adult animal. (D) Expression of DAF-2B::tdTomato with rgef-1p::GFP in neurons in a dauer.