Fig. 2.

Schematic illustration of the everted intestine model. (A) The small intestine, excluding the duodenum, was divided into three equal parts, and the distal part was excised and the length adjusted to 10 cm. The parts were then designated I, II, and III in distal order. In the same manner, the colon was excised and adjusted to 10 cm, taken from the distal end. (B) The everted intestine was affixed to a polyethylene tube. Serosal buffer was circulated at 5 ml/min using a pump. Zearalenone (10 µM) was added to the mucosal buffer. The water temperature was maintained at 37°C, and the tissue was aerated with 95% O₂/5% CO₂.