Table 3. Stability of sumatriptan standard in plasma under four different conditions (n=3).
| Storage condition | LQC (0.9 ng/mL) | HQC (80 ng/mL) | |||
|---|---|---|---|---|---|
| Testa | Referenceb | Testa | Referenceb | ||
| Post-preparation (24 h at 10 ℃) | Mean | 0.93 | 0.89 | 77.27 | 76.85 |
| SD (±) | 0.02 | 0.02 | 2.67 | 1.45 | |
| RSD (%) | 2.49 | 2.25 | 3.45 | 1.89 | |
| Freeze-thaw cycles (three cycles) | Mean | 0.90 | 0.90 | 72.84 | 71.86 |
| SD (±) | 0.03 | 0.05 | 1.80 | 0.84 | |
| RSD (%) | 2.94 | 5.03 | 2.48 | 1.16 | |
| Short-term (6 h at room temperature) | Mean | 0.90 | 0.93 | 76.31 | 76.85 |
| SD (±) | 0.03 | 0.02 | 0.49 | 1.67 | |
| RSD (%) | 3.38 | 1.86 | 0.64 | 2.18 | |
| Long-term (211 days at −80 ℃) | Mean | 0.97 | 0.94 | 83.40 | 83.66 |
| SD (±) | 0.01 | 0.01 | 1.18 | 0.53 | |
| RSD (%) | 0.59 | 1.23 | 1.41 | 0.63 | |
aConcentration of samples stored under different storage conditions; bConcentration of freshly prepared samples.