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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: FEBS Lett. 2019 Nov 16;594(4):646–664. doi: 10.1002/1873-3468.13648

Figure 7. Steady-State Kinetics of PKM2 “Literature” Mutants with Respect to PEP Concentration.

Figure 7.

All proteins were prepared using Prep 3. For each indicated mutant, activity was assessed with ADP concentration held constant at 2 mM. Assays were performed in duplicate and all data points are shown with allosteric sigmoidal (Hill) fit lines.