Table 2.

Michaelis-Menten Kinetic Parameters for ADP in the Presence and Absence of 2 mM FBP for Wild-Type and Mutant PKM2.

Enzyme	FBP	Km (μM)	Vmax (U/mg)	R2
PKM2 (Prep 3)	−	364.6 ± 33.20	209.6 ± 6.740	0.9909
	+	453.6 ± 29.40	189.8 ± 4.630	0.9957
PKM1	−	744.4 ± 62.40	153.1 ± 5.628	0.9940
	+	623.7 ± 37.24	168.4 ± 4.168	0.9967
PKM2 P117L	−	1836 ± 132.7	94.74 ± 4.013	0.9978
	+	1932 ± 109.0	169.6 ± 5.699	0.9987
PKM2 R246S	−	406.4 ± 90.97	124.0 ± 10.12	0.9511
	+	302.9 ± 26.98	133.5 ± 3.987	0.9905
PKM2 G415R	−	294.1 ± 20.84	83.96 ± 1.891	0.9949
	+	558.6 ± 81.54	103.2 ± 5.989	0.9820
PKM2 R455Q	−	495.7 ± 87.61	161.0 ± 10.99	0.9683
	+	426.7 ± 32.36	208.7 ± 5.848	0.9938
PKM2 R516C	−	45.75 ± 6.187	11.64 ± 0.3176	0.9718
	+	50.34 ± 9.253	12.82 ± 0.4903	0.9492
PKM2 S37A	−	338.0 ± 36.89	87.31 ± 3.294	0.9858
	+	459.4 ± 61.19	157.1 ± 7.904	0.9816
PKM2 K270M	−	275.7 ± 47.84	6.063 ± 0.3438	0.9644
	+	185.7 ± 72.53	5.909 ± 0.7172	0.8012
PKM2 K367M	−	274.7 ± 30.90	45.47 ± 1.670	0.9844
	+	297.4 ± 24.85	28.31 ± 0.7892	0.9911
PKM2 R399E	−	513.1 ± 34.49	172.5 ± 4.515	0.9956
	+	657.1 ± 71.43	166.4 ± 8.336	0.9903
PKM2 K433E	−	444.0 ± 80.40	113.8 ± 7.702	0.9656
	+	713.5 ± 31.26	191.6 ± 3.632	0.9984
PKM2 H464A	−	456.9 ± 36.59	138.6 ± 4.185	0.9933
	+	612.2 ± 42.74	213.1 ± 6.132	0.9957