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The N648Y mutation leads to reduced protein O‐GlcNAcylation in 3HA‐N648Y mES cells. (A) Immunoblots showing OGA, protein O‐GlcNAcylation (RL2) and OGT levels in 3HA‐WT and 3HA‐N648Y undifferentiated mES cells. (B) Quantification of western blotting of OGA, protein O‐GlcNAcylation (RL2) and OGT levels normalised to tubulin signal. n = 3, mean ± SD. Unpaired t‐test. ** corresponds to P = 0.0068 (RL2); *** corresponds to P = 0.0003 (OGA).
