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. 2020 Jan 26;177(5):1164–1186. doi: 10.1111/bph.14910

Figure 2.

Figure 2

The relaxant effects of GoSlo‐SR compounds are not abolished when BK channels are blocked. (a) Effect of GoSlo‐SR‐5‐130 on spontaneous tone of isobaric vessel preparations at 80 mmHg. Normalised vessel diameter (ratio of diameter/fully relaxed diameter at 80 mmHg) at different concentrations of GoSlo‐SR‐5‐130 in the absence (Control) and presence of 10−7 M IBTX (IBTX; repeated measures ANOVA: n = 6; P = 0.20); (b) vessel dilation in the absence (Time control = application of GoSlo‐SR solvent DMSO) and presence of GoSlo‐SR‐5‐130 (Control; repeated measures ANOVA: n = 6; P < .05), vessel dilation at different concentrations of GoSlo‐SR‐5‐130 in the absence (Control) and presence of 10−7 M IBTX (IBTX; repeated measures ANOVA: n = 6; P = 0.28); (c) effect of GoSlo‐SR‐5‐6 on spontaneous tone of isobaric vessel preparations at 80 mmHg. Normalised vessel diameter (ratio of diameter/fully relaxed diameter at 80 mmHg) at different concentrations of GoSlo‐SR‐5‐6 in the absence (Control) and presence of 10−7 M IBTX (IBTX; repeated measures ANOVA: n = 7; P < .05); (d) vessel dilation in the absence (Time control = application of GoSlo‐SR solvent DMSO) and presence of GoSlo‐SR‐5‐6 (Control; repeated measures ANOVA: n = 7; P < .05), vessel dilation at different concentrations of GoSlo‐SR‐5‐6 in the absence (Control) and presence of 10−7 M IBTX (IBTX; repeated measures ANOVA: n = 7; P = 0.94); (e) effect of GoSlo‐SR‐5‐6 on the pressure–diameter relationship of isobaric vessel preparations. Normalised vessel diameter (ratio of diameter/fully relaxed diameter at 80 mmHg) at different pressures in the absence of any substances (Control), in the presence of 10−6 M GoSlo‐SR‐5‐6, in the presence of 10−7 M IBTX and in the presence of both GoSlo‐SR‐5‐6 and IBTX (repeated measures ANOVA: n = 10; * P < .05); (f) effect of GoSlo‐SR‐5‐6 in the absence (Control) and presence of 10−7 M IBTX (unpaired Student's t‐ test: n = 10). The effect of GoSlo‐SR‐5‐6 in the absence of IBTX was quantified as (area under the GoSlo‐SR‐5‐6 curve) − (area under the control curve). The effect of GoSlo‐SR‐5‐6 in the presence of IBTX was quantified as (area under the GoSlo‐SR‐5‐6 + IBTX curve) − (area under the IBTX curve); (g) effect of IBTX in the absence (Control) and presence of 10−6 M GoSlo‐SR‐5‐6 (unpaired Student's t‐test: n = 10). The effect of IBTX in the absence of GoSlo‐SR‐5‐6 was quantified as (area under the control curve) − (area under the IBTX curve). The effect of IBTX in the presence of GoSlo‐SR‐5‐6 was quantified as (area under the GoSlo‐SR‐5‐6 curve) − (area under the GoSlo‐SR‐5‐6 + IBTX curve)