Figure 16.

GoSlo‐SR compounds activate K_v7.5 channels. (a) Typical family of currents obtained from a HEK cell during a series of voltage steps from −100 to +50 mV in 10‐mV increments lasting 1 s. Dotted lines represent the zero current level. Cells were held at −80 mV and repolarised back to −120 mV to obtain tail currents; (b) currents from the same cell during incubation with 10⁻⁵ M GoSlo‐SR‐5‐6; (c) summary activation curves obtained by measuring tail currents in six cells before (open circles) and during (blue circles) application of GoSlo‐SR‐5‐6; (d) and (e) currents obtained from a different cell, held at −80 mV and stepped from −100 to +50 mV in 10‐mV increments, in the absence and presence of GoSlo‐SR‐5‐130 (10⁻⁵ M), respectively. Tail current deactivation increased from 26 ± 4 ms to 46 ± 4 ms following a repolarisation from +40 to −120 mV (n = 5; P < .05; paired t test); (f) summary activation curves obtained from five cells in the absence (open circles) and presence (pink circles) of GoSlo‐SR‐5‐130