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rtPCR was performed to ensure that adequate knockdown (A) and overexpression (B) of U2AF65 and JMJD6 was achieved in HUVECs (N = 4). Western blots were also done to see if the message translated to the protein level. Western blots for knockdown of U2AF65 and JMJD6 are shown in (C) and samples from overexpression of the proteins are seen in (D) (N = 2). *P < 0.05; #P < 0.01
