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. 2020 Feb 25;40(2):BSR20193252. doi: 10.1042/BSR20193252

Figure 6. Validation of FLT1 primers.

Figure 6

BeWo and HUVEC samples were used to examine the validity of previously published and newly designed primers. The first five samples are from BeWo and in the following order: previously published Flt-1 (P. Flt-1), previously published sFlt-1 (P. sFlt-1), newly designed Flt-1 (Flt-1), newly designed sFlt-1 V2 (V2), newly designed sFlt-1 V3 (V3). The next five samples are from HUVEC and in the same order as BeWo. The previously published sFlt-1 appears to have multiple bands in BeWo samples, indicated by the white arrows, while it does not appear at all in HUVEC samples. HUVEC samples also do not seem to express much sFlt-1 V2, as our primer for it cannot be seen, despite having a clear band in BeWo samples.