Figure 2. Sappanone A (SA) pretreatment inhibited H/R-induced apoptosis.

H9c2 cardiomyocytes were treated by 25 µM SA for 1 h, followed by 6 h of hypoxia/3 h of reoxygenation. (A) Cell apoptosis was detected by flow cytometry. Cells in the lower right quadrant represent apoptosis cells. (B) Hoechst staining assay was performed to assess cell apoptosis in H9c2 cells. Hoechst-positive nuclei (apoptotic nuclei) showed dense and high-density fluorescence, as indicated by arrows, and the percentage of Hoechst-positive nuclei per optical field (at least 5 fields) was counted. Magnification: ×400, scale bars: 50 μm. Data are presented as the mean ± standard deviation from three independent experiments; * P < 0.05.