Figure 4. Sappanone A (SA) pretreatment inhibited mitochondrial apoptosis related proteins.

H9c2 cardiomyocytes were treated by 25 µM SA for 1 h, followed by 6 h of hypoxia/3 h of reoxygenation. The protein expression of cytochrome c in cytoplasm (A) or mitochondria (B) and the cleaved caspase-9 and caspase-3 (C) were detected by Western blotting. Data are presented as the mean ± standard deviation from three independent experiments; * P < 0.05.